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Service from UW Hospital to Fitchburg via Observatory, Babcock, Linden, Charter, University and Johnson, Broom and Bassett, John Nolen Drive, Rimrock, Highway MM, Brassica, Radicchio, Lacy, E Cheryl Parkway, Fish Hatchery, Lacy, and Research Park Drive.
Route 65 (Southbound)
- Begins northbound on University Bay
- To Highland
- Roundabout, 2nd right onto Observatory
- Right on Babcock
- Left on Linden
- Right on Charter
- Left on W Johnson
- Right on Bassett
- Left to W Wilson
- Right on Broom
- Right on John Nolen
- Right on Rimrock
- To County Rd MM
- Left on Lacy
- Left on Brassica
- Right on Radicchio
- Right on Lacy
- Cross US Hwy 14
- To Lacy
- Roundabout, 1st right to E Cheryl Pkwy
- Left on S Fish Hatchery
- Left on Lacy
- Left on Research Park Dr
- Ends northbound on Research Park Dr
Route 65 (Northbound)
- Starts northbound on Research Park Dr
- Right on E Cheryl Pkwy
- Roundabout, 2nd right to continue on E Cheryl Pkwy
- Roundabout 3rd right on Racy
- Left on Brassica
- Right on Radicchio
- Right on Lacy
- Right on County Rd MM
- To Rimrock
- Left on John Nolen
- Left on S Broom
- Left on Gorham
- To University Ave
- Right on N Charter
- Left on Linden
- Right on Babcock
- Left on Observatory
- Roundabout, 2nd right continues on Observatory
- Roundabout, 2nd right on Highland
- Right on Campus Ave ramp
- Right on University Bay
- Ends northbound on University Bay
