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Service from UW Hospital to Fitchburg via Observatory, Babcock, Linden, Charter, University and Johnson, Broom and Bassett, John Nolen Drive, Rimrock, Highway MM, Brassica, Radicchio, Lacy, E Cheryl Parkway, Fish Hatchery, Lacy, and Research Park Drive.

Route 65 (Southbound)

• Begins northbound on University Bay
• To Highland
• Roundabout, 2nd right onto Observatory
• Right on Babcock
• Left on Linden
• Right on Charter
• Left on W Johnson 
• Right on Bassett
• Left to W Wilson
• Right on Broom
• Right on John Nolen
• Right on Rimrock
• To County Rd MM
• Left on Lacy
• Left on Brassica
• Right on Radicchio
• Right on Lacy
• Cross US Hwy 14
• To Lacy
• Roundabout, 1st right to E Cheryl Pkwy
• Left on S Fish Hatchery
• Left on Lacy
• Left on Research Park Dr
• Ends northbound on Research Park Dr

Route 65 (Northbound)

• Starts northbound on Research Park Dr
• Right on E Cheryl Pkwy
• Roundabout, 2nd right to continue on E Cheryl Pkwy
• Roundabout 3rd right on Racy
• Left on Brassica
• Right on Radicchio
• Right on Lacy
• Right on County Rd MM
• To Rimrock
• Left on John Nolen
• Left on S Broom
• Left on Gorham
• To University Ave
• Right on N Charter
• Left on Linden
• Right on Babcock
• Left on Observatory
• Roundabout, 2nd right continues on Observatory
• Roundabout, 2nd right on Highland
• Right on Campus Ave ramp
• Right on University Bay
• Ends northbound on University Bay